KLF5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54921
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54921-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54921-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Krueppel-like factor 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human testis tissue with Krueppel-like factor 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of fixed and permeabilized human U-251 cells with Krueppel-like factor 5 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of human CCRF-CEM cell lysate with Krueppel-like factor 5 antibody. Predicted molecular weight: 34-51 kDa (multiple isoforms).
4 / 4
Flow cytometry testing of human HeLa cells with Krueppel-like factor 5 antibody; Blue=isotype control, Green= Krueppel-like factor 5 antibody.
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IHC testing of FFPE human testis tissue with Krueppel-like factor 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-251 cells with Krueppel-like factor 5 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human CCRF-CEM cell lysate with Krueppel-like factor 5 antibody. Predicted molecular weight: 34-51 kDa (multiple isoforms).
Flow cytometry testing of human HeLa cells with Krueppel-like factor 5 antibody; Blue=isotype control, Green= Krueppel-like factor 5 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50
Description:
KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 341-370 from the human protein was used as the immunogen for the Krueppel-like factor 5 antibody.
Limitation:
This Krueppel-like factor 5 antibody is available for research use only.
Localization:
Nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q13887