GIT1 Antibody / p95-APP1

NSJ Bioreagents
Product Code: NSJ-F54926
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54926-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54926-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the GIT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HeLa cell lysate with GIT1 antibody. Expected molecular weight: 84-95 kDa.
2 / 4
Western blot testing of human 1) U-2 OS and 2) HUVEC lysate with GIT1 antibody. Expected molecular weight: 84-95 kDa.
3 / 4
Western blot testing of mouse NIH 3T3 cell lysate with GIT1 antibody. Expected molecular weight: 84-95 kDa.
4 / 4
Flow cytometry testing of human NCI-H460 cells with GIT1 antibody; Blue=isotype control, Green= GIT1 antibody.

Western blot testing of human HeLa cell lysate with GIT1 antibody. Expected molecular weight: 84-95 kDa.
Western blot testing of human 1) U-2 OS and 2) HUVEC lysate with GIT1 antibody. Expected molecular weight: 84-95 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with GIT1 antibody. Expected molecular weight: 84-95 kDa.
Flow cytometry testing of human NCI-H460 cells with GIT1 antibody; Blue=isotype control, Green= GIT1 antibody.

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:50-1:100 (1x10e6 cells)
Description:
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 533-561 from the human protein was used as the immunogen for the GIT1 antibody.
Limitation:
This GIT1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9Y2X7