Vinculin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54927
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54927-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54927-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the VCL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human RD, 2) human HeLa, 3) rat liver and 4) human testis lysate with VCL antibody. Predicted molecular weight ~124 kDa.
2 / 4
Western blot testing of human K562 cell lysate with VCL antibody. Predicted molecular weight ~124 kDa.
3 / 4
Western blot testing of mouse lung tissue lysate with VCL antibody. Predicted molecular weight ~124 kDa.
4 / 4
IHC testing of FFPE human skeletal muscle tissue with VCL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) human RD, 2) human HeLa, 3) rat liver and 4) human testis lysate with VCL antibody. Predicted molecular weight ~124 kDa.
Western blot testing of human K562 cell lysate with VCL antibody. Predicted molecular weight ~124 kDa.
Western blot testing of mouse lung tissue lysate with VCL antibody. Predicted molecular weight ~124 kDa.
IHC testing of FFPE human skeletal muscle tissue with VCL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:10-1:50
Description:
VCL/Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 12-39 from the human protein was used as the immunogen for the VCL antibody.
Limitation:
This VCL antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P18206