Vitronectin Antibody / VTN

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NSJ Bioreagents
Product Code: NSJ-F54930
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54930-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54930-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Vitronectin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with Vitronectin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human Caco-2 cell lysate with Vitronectin antibody. Expected molecular weight: 54-75 kDa depending on glycosylation level.
3 / 4
Western blot testing of human plasma lysate with Vitronectin antibody. Expected molecular weight: 54-75 kDa depending on glycosylation level.
4 / 4
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Vitronectin antibody; Blue=isotype control, Green= Vitronectin antibody.
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IHC testing of FFPE human lung carcinoma tissue with Vitronectin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human Caco-2 cell lysate with Vitronectin antibody. Expected molecular weight: 54-75 kDa depending on glycosylation level.
Western blot testing of human plasma lysate with Vitronectin antibody. Expected molecular weight: 54-75 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Vitronectin antibody; Blue=isotype control, Green= Vitronectin antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:1000-1:2000, Flow cytometry: 1:25 (1x10e6 cells)
Description:
Vitronectin is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 65-93 from the human protein was used as the immunogen for the Vitronectin antibody.
Limitation:
This Vitronectin antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P04004