GCAT Antibody / Glycine acetyltransferase

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NSJ Bioreagents
Product Code: NSJ-F54934
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54934-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54934-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GCAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with GCAT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GCAT antibody.
3 / 4
Western blot testing of mouse liver tissue lysate with GCAT antibody. Predicted molecular weight ~45 kDa.
4 / 4
Flow cytometry testing of human HepG2 cells with GCAT antibody; Blue=isotype control, Green= GCAT antibody.
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IHC testing of FFPE human brain tissue with GCAT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GCAT antibody.
Western blot testing of mouse liver tissue lysate with GCAT antibody. Predicted molecular weight ~45 kDa.
Flow cytometry testing of human HepG2 cells with GCAT antibody; Blue=isotype control, Green= GCAT antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 155-181 from the human protein was used as the immunogen for the GCAT antibody.
Limitation:
This GCAT antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
O75600