p21 Antibody / CDKN1A
| Code | Size | Price |
|---|
| NSJ-F54937-0.08ML | 0.08 ml | £260.00 |
Quantity:
| NSJ-F54937-0.4ML | 0.4 ml | £502.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Aliquot the p21WAF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Description:
CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 134-164 from the human protein was used as the immunogen for the p21WAF1 antibody.
Limitation:
This p21WAF1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P38936