SYK Antibody / Spleen tyrosine kinase

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NSJ Bioreagents
Product Code: NSJ-F54945
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54945-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54945-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Spleen tyrosine kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human Raji cell lysate with Spleen tyrosine kinase antibody. Predicted molecular weight ~72 kDa.
2 / 4
Western blot testing of 1) human ThP-1 and 2) mouse spleen tissue lysate with Spleen tyrosine kinase antibody. Predicted molecular weight ~72 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Spleen tyrosine kinase antibody.
4 / 4
IHC testing of FFPE human cancer tissue with Spleen tyrosine kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human Raji cell lysate with Spleen tyrosine kinase antibody. Predicted molecular weight ~72 kDa.
Western blot testing of 1) human ThP-1 and 2) mouse spleen tissue lysate with Spleen tyrosine kinase antibody. Predicted molecular weight ~72 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Spleen tyrosine kinase antibody.
IHC testing of FFPE human cancer tissue with Spleen tyrosine kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 387-417 from the human protein was used as the immunogen for the Spleen tyrosine kinase antibody.
Limitation:
This Spleen tyrosine kinase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P43405