Filamin A Antibody / FLNA

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NSJ Bioreagents
Product Code: NSJ-F54947
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54947-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54947-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Filamin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skeletal muscle tissue with Filamin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with Filamin A antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human U-251 cell lysate with Filamin A antibody. Predicted molecular weight ~281 kDa.
4 / 4
Flow cytometry testing of human U-251 cells with Filamin A antibody; Left=isotype control, Right= Filamin A antibody.
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IHC testing of FFPE human skeletal muscle tissue with Filamin A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Filamin A antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human U-251 cell lysate with Filamin A antibody. Predicted molecular weight ~281 kDa.
Flow cytometry testing of human U-251 cells with Filamin A antibody; Left=isotype control, Right= Filamin A antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Description:
Actin-binding protein 280, or filamin, is a 280-kD protein that crosslinks actin filaments into orthogonal networks in cortical cytoplasm and participates in the anchoring of membrane proteins for the actin cytoskeleton. Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1025-1054 from the human protein was used as the immunogen for the Filamin A antibody.
Limitation:
This Filamin A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P21333