NGF Antibody / Beta Nerve Growth Factor

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NSJ Bioreagents
Product Code: NSJ-F54948
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54948-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54948-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Beta-nerve growth factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with Beta-nerve growth factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human MDA-MB-231 cells with Beta-nerve growth factor antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human NCI-H460 cell lysate with Beta-nerve growth factor antibody. Predicted molecular weight ~27 kDa.
4 / 4
Flow cytometry testing of human NCI-H460 cells with Beta-nerve growth factor antibody; Blue=isotype control, Green= Beta-nerve growth factor antibody.
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IHC testing of FFPE human brain tissue with Beta-nerve growth factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MDA-MB-231 cells with Beta-nerve growth factor antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human NCI-H460 cell lysate with Beta-nerve growth factor antibody. Predicted molecular weight ~27 kDa.
Flow cytometry testing of human NCI-H460 cells with Beta-nerve growth factor antibody; Blue=isotype control, Green= Beta-nerve growth factor antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Description:
NGFB is a member of the NGF-beta family. It is a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in the gene encoding NGFB have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of the gene's expression is associated with allergic rhinitis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 84-115 from the human protein was used as the immunogen for the Beta-nerve growth factor antibody.
Limitation:
This Beta-nerve growth factor antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P01138