Cytochrome P450 3A5 Antibody / CYP3A5

Contact us
NSJ Bioreagents
Product Code: NSJ-F54950
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54950-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54950-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cytochrome P450 3A5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human SK-BR-3 cell lysate with Cytochrome P450 3A5 antibody. Predicted molecular weight ~57 kDa.
2 / 4
Western blot testing of human HEK293 cell lysate with Cytochrome P450 3A5 antibody. Predicted molecular weight ~57 kDa.
3 / 4
Immunofluorescent staining of human HEK293 cells with Cytochrome P450 3A5 antibody (green) and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with Cytochrome P450 3A5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
❮❯

Western blot testing of human SK-BR-3 cell lysate with Cytochrome P450 3A5 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of human HEK293 cell lysate with Cytochrome P450 3A5 antibody. Predicted molecular weight ~57 kDa.
Immunofluorescent staining of human HEK293 cells with Cytochrome P450 3A5 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human hepatocellular carcinoma tissue with Cytochrome P450 3A5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50
Description:
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 476-502 from the human protein was used as the immunogen for the Cytochrome P450 3A5 antibody.
Limitation:
This Cytochrome P450 3A5 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P20815