CYP21A2 Antibody / Steroid 21-hydroxylase

NSJ Bioreagents
Product Code: NSJ-F54956
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54956-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54956-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Steroid 21-hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human breast carcinoma tissue with Steroid 21-hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HEK293 cells with Steroid 21-hydroxylase antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human MDA-MB-435 cell lysate with Steroid 21-hydroxylase antibody. Expected molecular weight: 52-56 kDa.
4 / 4
Flow cytometry testing of human MDA-MB-231 cells with Steroid 21-hydroxylase antibody; Blue=isotype control, Green= Steroid 21-hydroxylase antibody.

IHC testing of FFPE human breast carcinoma tissue with Steroid 21-hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK293 cells with Steroid 21-hydroxylase antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human MDA-MB-435 cell lysate with Steroid 21-hydroxylase antibody. Expected molecular weight: 52-56 kDa.
Flow cytometry testing of human MDA-MB-231 cells with Steroid 21-hydroxylase antibody; Blue=isotype control, Green= Steroid 21-hydroxylase antibody.

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Description:
CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 216-246 from the human protein was used as the immunogen for the Steroid 21-hydroxylase antibody.
Limitation:
This Steroid 21-hydroxylase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P08686