PI3KCD Antibody / PI3K delta / p110 delta

NSJ Bioreagents
Product Code: NSJ-F54959
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54959-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54959-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PI3K delta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HT-1080 cell lysate with PI3K delta antibody. Expected molecular weight: 110-120 kDa.
2 / 4
Western blot testing of human Raji cell lysate with PI3K delta antibody. Expected molecular weight: 110-120 kDa.
3 / 4
IHC testing of FFPE human epityphlon tissue with PI3K delta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PI3K delta antibody (green) and anti-Actin (red).

Western blot testing of human HT-1080 cell lysate with PI3K delta antibody. Expected molecular weight: 110-120 kDa.
Western blot testing of human Raji cell lysate with PI3K delta antibody. Expected molecular weight: 110-120 kDa.
IHC testing of FFPE human epityphlon tissue with PI3K delta antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PI3K delta antibody (green) and anti-Actin (red).

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 687-717 from the human protein was used as the immunogen for the PI3K delta antibody.
Limitation:
This PI3K delta antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O00329