PCK2 Antibody / PEPCK2

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NSJ Bioreagents
Product Code: NSJ-F54964
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54964-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54964-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PEPCK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HepG2 cell lysate with PEPCK2 antibody. Predicted molecular weight ~71 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PEPCK2 antibody.
3 / 4
Immunofluorescent staining of human HepG2 cells with PEPCK2 antibody (green) and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human breast cancer tissue with PEPCK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HepG2 cell lysate with PEPCK2 antibody. Predicted molecular weight ~71 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PEPCK2 antibody.
Immunofluorescent staining of human HepG2 cells with PEPCK2 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human breast cancer tissue with PEPCK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-54 from the human protein was used as the immunogen for the PEPCK2 antibody.
Limitation:
This PEPCK2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q16822