Fructose 6 Phosphate Kinase Antibody / PFKM

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NSJ Bioreagents
Product Code: NSJ-F54966
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54966-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54966-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PFKM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HeLa cell lysate with PFKM antibody. Predicted molecular weight ~85 kDa.
2 / 4
Immunofluorescent staining of human HeLa cells with PFKM antibody (green) and DAPI nuclear stain (blue).
3 / 4
IHC testing of FFPE human skeletal muscle tissue with PFKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with PFKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HeLa cell lysate with PFKM antibody. Predicted molecular weight ~85 kDa.
Immunofluorescent staining of human HeLa cells with PFKM antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human skeletal muscle tissue with PFKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human hepatocellular carcinoma tissue with PFKM antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 746-776 from the human protein was used as the immunogen for the PFKM antibody.
Limitation:
This PFKM antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P08237