14-3-3 beta/alpha Antibody / YWHAB

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NSJ Bioreagents
Product Code: NSJ-F54967
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54967-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54967-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the YWHAB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human Jurkat cell lysate with YWHAB antibody. Predicted molecular weight ~28 kDa.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with YWHAB antibody.
3 / 4
Immunofluorescent staining of human HeLa cells with YWHAB antibody (green) and DAPI nuclear stain (blue).
4 / 4
IHC testing of FFPE human breast cancer tissue with YWHAB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human Jurkat cell lysate with YWHAB antibody. Predicted molecular weight ~28 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with YWHAB antibody.
Immunofluorescent staining of human HeLa cells with YWHAB antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human breast cancer tissue with YWHAB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 217-246 from the human protein was used as the immunogen for the YWHAB antibody.
Limitation:
This YWHAB antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P31946