Calmodulin Antibody / CALM1

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NSJ Bioreagents
Product Code: NSJ-F54975
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54975-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54975-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CALM1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HeLa cell lysate with CALM1 antibody. Predicted molecular weight ~17 kDa.
2 / 4
IHC testing of FFPE human brain tissue with CALM1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HeLa cells with CALM1 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human HeLa cells with CALM1 antibody; Blue=isotype control, Green= CALM1 antibody.
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Western blot testing of human HeLa cell lysate with CALM1 antibody. Predicted molecular weight ~17 kDa.
IHC testing of FFPE human brain tissue with CALM1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with CALM1 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HeLa cells with CALM1 antibody; Blue=isotype control, Green= CALM1 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000
Description:
CALM1/Calmodulin is a member of the EF-hand calcium-binding protein family. Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 107-132 from the human protein was used as the immunogen for the CALM1 antibody.
Limitation:
This CALM1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P0DP23