ADRA2B Antibody / Alpha-2B Adrenergic Receptor

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NSJ Bioreagents
Product Code: NSJ-F54979
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54979-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54979-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Alpha-2B Adrenergic Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) MCF7, 2) MDA-MB-231 and 3) SK-BR-3 cell lysate with Alpha-2B Adrenergic Receptor antibody. Predicted molecular weight ~50 kDa.
2 / 4
Immunofluorescent staining of fixed human MDA-MB-231 cells with Alpha-2B Adrenergic Receptor antibody (green) and DAPI nuclear stain (blue).
3 / 4
IHC testing of FFPE human breast cancer tissue with Alpha-2B Adrenergic Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MDA-MB-231 cells with Alpha-2B Adrenergic Receptor antibody; Blue=isotype control, Green= Alpha-2B Adrenergic Receptor antibody.
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Western blot testing of human 1) MCF7, 2) MDA-MB-231 and 3) SK-BR-3 cell lysate with Alpha-2B Adrenergic Receptor antibody. Predicted molecular weight ~50 kDa.
Immunofluorescent staining of fixed human MDA-MB-231 cells with Alpha-2B Adrenergic Receptor antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human breast cancer tissue with Alpha-2B Adrenergic Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-231 cells with Alpha-2B Adrenergic Receptor antibody; Blue=isotype control, Green= Alpha-2B Adrenergic Receptor antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 343-369 from the human protein was used as the immunogen for the Alpha-2B Adrenergic Receptor antibody.
Limitation:
This Alpha-2B Adrenergic Receptor antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P18089