PRKCA Antibody / PKC alpha

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NSJ Bioreagents
Product Code: NSJ-F54980
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54980-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54980-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PKC alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human lung carcinoma tissue with PKC alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with PKC alpha antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of mouse NIH 3T3 cell lysate with PKC alpha antibody. Predicted molecular weight ~77 kDa.
4 / 4
Flow cytometry testing of human CEM cells with PKC alpha antibody; Blue=isotype control, Green= PKC alpha antibody.
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IHC testing of FFPE human lung carcinoma tissue with PKC alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with PKC alpha antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of mouse NIH 3T3 cell lysate with PKC alpha antibody. Predicted molecular weight ~77 kDa.
Flow cytometry testing of human CEM cells with PKC alpha antibody; Blue=isotype control, Green= PKC alpha antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Description:
PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 75-103 from the human protein was used as the immunogen for the PKC alpha antibody.
Limitation:
This PKC alpha antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P17252