4-hydroxyphenylpyruvate dioxygenase Antibody / 4HPPD / HPD

NSJ Bioreagents
Product Code: NSJ-F54982
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54982-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54982-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HPD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of mouse liver tissue lysate with HPD antibody. Predicted molecular weight ~45 kDa.
2 / 4
Western blot testing of human HepG2 cell lysate with HPD antibody. Predicted molecular weight ~45 kDa.
3 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with HPD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with HPD antibody; Blue=isotype control, Green= HPD antibody.

Western blot testing of mouse liver tissue lysate with HPD antibody. Predicted molecular weight ~45 kDa.
Western blot testing of human HepG2 cell lysate with HPD antibody. Predicted molecular weight ~45 kDa.
IHC testing of FFPE human hepatocellular carcinoma tissue with HPD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with HPD antibody; Blue=isotype control, Green= HPD antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
HPD is an enzyme in the catabolic pathway of tyrosine. This protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 92-121 from the human protein was used as the immunogen for the HPD antibody.
Limitation:
This HPD antibody is available for research use only.
Localization:
Cytoplasmic, extracellular
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P32754