ARPC1B Antibody

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NSJ Bioreagents
Product Code: NSJ-F54999
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54999-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54999-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ARPC1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with ARPC1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HeLa cell lysate with ARPC1B antibody. Predicted molecular weight ~41 kDa.
3 / 4
Western blot testing of human A431 cell lysate with ARPC1B antibody. Predicted molecular weight ~41 kDa.
4 / 4
Flow cytometry testing of human MCF7 cells with ARPC1B antibody; Blue=isotype control, Green= ARPC1B antibody.
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IHC testing of FFPE human lung carcinoma tissue with ARPC1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with ARPC1B antibody. Predicted molecular weight ~41 kDa.
Western blot testing of human A431 cell lysate with ARPC1B antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of human MCF7 cells with ARPC1B antibody; Blue=isotype control, Green= ARPC1B antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
ARPC1B is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1A. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 159-188 from the human protein was used as the immunogen for the ARPC1B antibody.
Limitation:
This ARPC1B antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O15143