CDC42EP3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F55000
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55000-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55000-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CDC42EP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human testis tissue with CDC42EP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HEK293 cell lysate with CDC42EP3 antibody. Predicted molecular weight ~28 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with CDC42EP3 antibody.
4 / 4
Flow cytometry testing of human HEK293 cells with CDC42EP3 antibody; Blue=isotype control, Green= CDC42EP3 antibody.
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IHC testing of FFPE human testis tissue with CDC42EP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HEK293 cell lysate with CDC42EP3 antibody. Predicted molecular weight ~28 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with CDC42EP3 antibody.
Flow cytometry testing of human HEK293 cells with CDC42EP3 antibody; Blue=isotype control, Green= CDC42EP3 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
CDC42EP3 is probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. It induces pseudopodia formation in fibroblasts.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the CDC42EP3 antibody.
Limitation:
This CDC42EP3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UKI2