PPID Antibody / Cyclophilin D

NSJ Bioreagents
Product Code: NSJ-F55003
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55003-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55003-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PPIase D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse NIH 3T3 cell lysate with PPIase D antibody. Predicted molecular weight ~41 kDa.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PPIase D antibody.
3 / 4
IHC testing of FFPE human brain tissue with PPIase D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human K562 cells with PPIase D antibody; Blue=isotype control, Green= PPIase D antibody.

Western blot testing of mouse NIH 3T3 cell lysate with PPIase D antibody. Predicted molecular weight ~41 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PPIase D antibody.
IHC testing of FFPE human brain tissue with PPIase D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with PPIase D antibody; Blue=isotype control, Green= PPIase D antibody.

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Description:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 85-115 from the human protein was used as the immunogen for the PPIase D antibody.
Limitation:
This PPIase D antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q08752