COTL1 Antibody / Coactosin-like protein

Contact us
NSJ Bioreagents
Product Code: NSJ-F55004
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55004-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55004-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the COTL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human spleen tissue with COTL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HeLa cell lysate with COTL1 antibody. Predicted molecular weight ~16 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with COTL1 antibody.
4 / 4
Flow cytometry testing of human HeLa cells with COTL1 antibody; Blue=isotype control, Green= COTL1 antibody.
❮❯

IHC testing of FFPE human spleen tissue with COTL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with COTL1 antibody. Predicted molecular weight ~16 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with COTL1 antibody.
Flow cytometry testing of human HeLa cells with COTL1 antibody; Blue=isotype control, Green= COTL1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 115-142 from the human protein was used as the immunogen for the COTL1 antibody.
Limitation:
This COTL1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q14019