Haptoglobin Antibody

NSJ Bioreagents
Product Code: NSJ-F55010
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55010-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55010-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Haptoglobin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human liver tissue lysate with Haptoglobin antibody. Predicted molecular weight: 45, 38 kDa (two isoforms).
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Haptoglobin antibody.
3 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with Haptoglobin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with Haptoglobin antibody; Blue=isotype control, Green= Haptoglobin antibody.

Western blot testing of human liver tissue lysate with Haptoglobin antibody. Predicted molecular weight: 45, 38 kDa (two isoforms).
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Haptoglobin antibody.
IHC testing of FFPE human hepatocellular carcinoma tissue with Haptoglobin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with Haptoglobin antibody; Blue=isotype control, Green= Haptoglobin antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
HP is a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 295-322 from the human protein was used as the immunogen for the Haptoglobin antibody.
Limitation:
This Haptoglobin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P00738