RAB8A Antibody

NSJ Bioreagents
Product Code: NSJ-F55011
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55011-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55011-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the RAB8A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HCT-116 cell lysate with RAB8A antibody. Predicted molecular weight ~24 kDa.
2 / 4
Western blot testing of human HeLa cell lysate with RAB8A antibody. Predicted molecular weight ~24 kDa.
3 / 4
IHC testing of FFPE human brain tissue with RAB8A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HeLa cells with RAB8A antibody; Blue=isotype control, Green= RAB8A antibody.

Western blot testing of human HCT-116 cell lysate with RAB8A antibody. Predicted molecular weight ~24 kDa.
Western blot testing of human HeLa cell lysate with RAB8A antibody. Predicted molecular weight ~24 kDa.
IHC testing of FFPE human brain tissue with RAB8A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with RAB8A antibody; Blue=isotype control, Green= RAB8A antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Description:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Anessential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. RAB8A is a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the RAB8A antibody.
Limitation:
This RAB8A antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P61006