Cdc37 Antibody

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NSJ Bioreagents
Product Code: NSJ-F55012
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55012-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55012-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cdc37 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human A431, 2) rat C6, 3) human Jurkat, 4) human K562 and 5) human LNCaP cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
2 / 4
Western blot testing of human 1) MCF7 and 2) A2058 cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
3 / 4
IHC testing of FFPE human skin tissue with Cdc37 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MCF7 cells with Cdc37 antibody; Blue=isotype control, Green= Cdc37 antibody.
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Western blot testing of 1) human A431, 2) rat C6, 3) human Jurkat, 4) human K562 and 5) human LNCaP cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
Western blot testing of human 1) MCF7 and 2) A2058 cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.
IHC testing of FFPE human skin tissue with Cdc37 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MCF7 cells with Cdc37 antibody; Blue=isotype control, Green= Cdc37 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:1000-1:2000
Description:
Cdc37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 116-144 from the human protein was used as the immunogen for the Cdc37 antibody.
Limitation:
This Cdc37 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
Q16543