Clathrin Light Chain A Antibody

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NSJ Bioreagents
Product Code: NSJ-F55014
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55014-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55014-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Clathrin Light Chain A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with Clathrin Light Chain A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Clathrin Light Chain A antibody.
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Western blot testing of human MDA-MB-231 cell lysate with Clathrin Light Chain A antibody. Expected molecular weight: 24-27 kDa.
4 / 4
Flow cytometry testing of human MDA-MB-231 cells with Clathrin Light Chain A antibody; Blue=isotype control, Green= Clathrin Light Chain A antibody.
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IHC testing of FFPE human lung carcinoma tissue with Clathrin Light Chain A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Clathrin Light Chain A antibody.
Western blot testing of human MDA-MB-231 cell lysate with Clathrin Light Chain A antibody. Expected molecular weight: 24-27 kDa.
Flow cytometry testing of human MDA-MB-231 cells with Clathrin Light Chain A antibody; Blue=isotype control, Green= Clathrin Light Chain A antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Description:
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 99-127 from the human protein was used as the immunogen for the Clathrin Light Chain A antibody.
Limitation:
This Clathrin Light Chain A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P09496