POU2AF1 Antibody / BOB-1

NSJ Bioreagents
Product Code: NSJ-F55017
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55017-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55017-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the POU2AF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Flow cytometry testing of human Ramos cells with POU2AF1 antibody; Blue=isotype control, Green= POU2AF1 antibody.
2 / 3
Western blot testing of human Ramos cell lysate with POU2AF1 antibody. Expected molecular weight: ~28/35-40 kDa (unmodified/ubiquitinated).
3 / 3
IHC testing of FFPE human tonsil tissue with POU2AF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry testing of human Ramos cells with POU2AF1 antibody; Blue=isotype control, Green= POU2AF1 antibody.
Western blot testing of human Ramos cell lysate with POU2AF1 antibody. Expected molecular weight: ~28/35-40 kDa (unmodified/ubiquitinated).
IHC testing of FFPE human tonsil tissue with POU2AF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:1000-1:2000
Description:
POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-27 from the human protein was used as the immunogen for the POU2AF1 antibody.
Limitation:
This POU2AF1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q16633