TIMP3 Antibody

NSJ Bioreagents
Product Code: NSJ-F55019
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55019-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55019-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TIMP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human breast carcinoma tissue with TIMP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HepG2 cell lysate with TIMP3 antibody. Expected molecular weight: 24-33 kDa depending on glycosylation level.
3 / 4
Western blot testing of mouse NIH 3T3 cell lysate, in the 1) absence and 2) presence of immunizing peptide, with TIMP3 antibody.
4 / 4
Flow cytometry testing of human MCF7 cells with TIMP3 antibody; Blue=isotype control, Green= TIMP3 antibody.

IHC testing of FFPE human breast carcinoma tissue with TIMP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with TIMP3 antibody. Expected molecular weight: 24-33 kDa depending on glycosylation level.
Western blot testing of mouse NIH 3T3 cell lysate, in the 1) absence and 2) presence of immunizing peptide, with TIMP3 antibody.
Flow cytometry testing of human MCF7 cells with TIMP3 antibody; Blue=isotype control, Green= TIMP3 antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Description:
TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 113-139 from the human protein was used as the immunogen for the TIMP3 antibody.
Limitation:
This TIMP3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P35625