DEK Antibody

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NSJ Bioreagents
Product Code: NSJ-F55024
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55024-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55024-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DEK antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) U-251 MG and 2) PC-3 cell lysate with DEK antibody. Expected molecular weight: 43-50 kDa.
2 / 4
IHC testing of FFPE human testis tissue with DEK antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HeLa cells with DEK antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human WiDr cells with DEK antibody; Blue=isotype control, Green= DEK antibody.
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Western blot testing of human 1) U-251 MG and 2) PC-3 cell lysate with DEK antibody. Expected molecular weight: 43-50 kDa.
IHC testing of FFPE human testis tissue with DEK antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with DEK antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human WiDr cells with DEK antibody; Blue=isotype control, Green= DEK antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 336-364 from the human protein was used as the immunogen for the DEK antibody.
Limitation:
This DEK antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P35659