Ataxin 3 Antibody / ATXN3

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NSJ Bioreagents
Product Code: NSJ-F55027
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55027-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55027-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Ataxin 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with Ataxin 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human MCF7 cell lysate with Ataxin 3 antibody. Predicted molecular weight ~42 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Ataxin 3 antibody.
4 / 4
Flow cytometry testing of human ZR-75-1 cells with Ataxin 3 antibody; Blue=isotype control, Green= Ataxin 3 antibody.
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IHC testing of FFPE human brain tissue with Ataxin 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human MCF7 cell lysate with Ataxin 3 antibody. Predicted molecular weight ~42 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Ataxin 3 antibody.
Flow cytometry testing of human ZR-75-1 cells with Ataxin 3 antibody; Blue=isotype control, Green= Ataxin 3 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
ATXN3 was known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 261-288 from the human protein was used as the immunogen for the Ataxin 3 antibody.
Limitation:
This Ataxin 3 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P54252