Thyroxine 5-deiodinase Antibody / DIO3

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NSJ Bioreagents
Product Code: NSJ-F55036
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55036-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55036-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Thyroxine 5-deiodinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human placental tissue lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
2 / 4
Western blot testing of human HepG2 cell lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
3 / 4
Western blot testing of rat skeletal muscle tissue lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
4 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with Thyroxine 5-deiodinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human placental tissue lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
Western blot testing of human HepG2 cell lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
Western blot testing of rat skeletal muscle tissue lysate with Thyroxine 5-deiodinase antibody. Predicted molecular weight ~34 kDa, routinely observed at 31-37 kDa.
IHC testing of FFPE human hepatocellular carcinoma tissue with Thyroxine 5-deiodinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:1000-1:2000, IHC, Immunohistochemistry (FFPE): 1:50-1:100
Description:
DIO3 / Thyroxine 5-deiodinase belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 250-278 from the human protein was used as the immunogen for the Thyroxine 5-deiodinase antibody.
Limitation:
This Thyroxine 5-deiodinase antibody is available for research use only.
Localization:
Cytoplasmic, cell membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P55073