ADH7 Antibody

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NSJ Bioreagents
Product Code: NSJ-F55038
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55038-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55038-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ADH7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HepG2 and 2) SW480 cell lysate with ADH7 antibody. Predicted molecular weight ~41 kDa.
2 / 4
IHC testing of FFPE human lung tissue with ADH7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human NCI-H460 cells with ADH7 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human K562 cells with ADH7 antibody; Blue=isotype control, Green= ADH7 antibody.
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Western blot testing of human 1) HepG2 and 2) SW480 cell lysate with ADH7 antibody. Predicted molecular weight ~41 kDa.
IHC testing of FFPE human lung tissue with ADH7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human NCI-H460 cells with ADH7 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human K562 cells with ADH7 antibody; Blue=isotype control, Green= ADH7 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100
Description:
This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 318-346 from the human protein was used as the immunogen for the ADH7 antibody.
Limitation:
This ADH7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P40394