ANKH Antibody

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NSJ Bioreagents
Product Code: NSJ-F55041
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55041-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55041-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ANKH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse cerebellum tissue lysate with ANKH antibody. Predicted molecular weight ~54 kDa.
2 / 4
Western blot testing of human U-2 OS cell lysate with ANKH antibody. Predicted molecular weight ~54 kDa.
3 / 4
IHC testing of FFPE human brain tissue with ANKH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human K562 cells with ANKH antibody; Blue=isotype control, Green= ANKH antibody.
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Western blot testing of mouse cerebellum tissue lysate with ANKH antibody. Predicted molecular weight ~54 kDa.
Western blot testing of human U-2 OS cell lysate with ANKH antibody. Predicted molecular weight ~54 kDa.
IHC testing of FFPE human brain tissue with ANKH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with ANKH antibody; Blue=isotype control, Green= ANKH antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Description:
ANKH is a multipass transmembrane protein that is expressed in joints and other tissues and controls pyrophosphate levels in cultured cells. Progressive ankylosis-mediated control of pyrophosphate levels has been suggested as a possible mechanism regulating tissue calcification and susceptibility to arthritis in higher animals.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 464-492 from the human protein was used as the immunogen for the ANKH antibody.
Limitation:
This ANKH antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9HCJ1