DFFB Antibody

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NSJ Bioreagents
Product Code: NSJ-F55043
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55043-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55043-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DFFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human prostate tissue with DFFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human 1) HeLa, 2) HepG2, 3) Jukat, 4) LNCaP and 5) Raji cell lysate with DFFB antibody. Predicted molecular weight ~39 kDa.
3 / 3
Flow cytometry testing of human HEK293 cells with DFFB antibody; Blue=isotype control, Green= DFFB antibody.
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IHC testing of FFPE human prostate tissue with DFFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) HepG2, 3) Jukat, 4) LNCaP and 5) Raji cell lysate with DFFB antibody. Predicted molecular weight ~39 kDa.
Flow cytometry testing of human HEK293 cells with DFFB antibody; Blue=isotype control, Green= DFFB antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50
Description:
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the DFFB antibody.
Limitation:
This DFFB antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O76075