PLA2G6 Antibody / CaI-PLA2

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NSJ Bioreagents
Product Code: NSJ-F55050
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55050-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55050-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PLA2G6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human testis carcinoma tissue with CaI-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Immunofluorescent staining of human HepG2 cells with CaI-PLA2 antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of human HepG2 cell lysate with CaI-PLA2 antibody. Expected molecular weight: 85-90 kDa.
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Flow cytometry testing of human HepG2 cells with CaI-PLA2 antibody; Blue=isotype control, Green= CaI-PLA2 antibody.
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IHC testing of FFPE human testis carcinoma tissue with CaI-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HepG2 cells with CaI-PLA2 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HepG2 cell lysate with CaI-PLA2 antibody. Expected molecular weight: 85-90 kDa.
Flow cytometry testing of human HepG2 cells with CaI-PLA2 antibody; Blue=isotype control, Green= CaI-PLA2 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Description:
The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 551-580 from the human protein was used as the immunogen for the CaI-PLA2 antibody.
Limitation:
This CaI-PLA2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O60733