DNAJC6 Antibody

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NSJ Bioreagents
Product Code: NSJ-F55053
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F55053-0.08ML0.08 ml£260.00
Quantity:
NSJ-F55053-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DNAJC6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human K562, 2) human HL60 and 3) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
2 / 4
Western blot testing of 1) human K562, 2) human SH-SY-5Y, 3) human U-87 MG, 4) human HL60, 5) mouse cerebellum, 6) mouse brain and 7) rat cerebellum lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
3 / 4
IHC testing of FFPE human brain tissue with DNAJC6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human K562 cells with DNAJC6 antibody; Blue=isotype control, Green= DNAJC6 antibody.
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Western blot testing of 1) human K562, 2) human HL60 and 3) mouse brain lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
Western blot testing of 1) human K562, 2) human SH-SY-5Y, 3) human U-87 MG, 4) human HL60, 5) mouse cerebellum, 6) mouse brain and 7) rat cerebellum lysate with DNAJC6 antibody. Predicted molecular weight ~100 kDa.
IHC testing of FFPE human brain tissue with DNAJC6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with DNAJC6 antibody; Blue=isotype control, Green= DNAJC6 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:10-1:50
Description:
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 254-281 from the human protein was used as the immunogen for the DNAJC6 antibody.
Limitation:
This DNAJC6 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O75061