ACTH Antibody / Synacthen

NSJ Bioreagents
Product Code: NSJ-V8807
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8807-20UG20ug£264.00
Quantity:
NSJ-V8807-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rCLIP/3814
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant ACTH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC staining of FFPE human pituitary gland tissue with recombinant ACTH antibody (clone rCLIP/3814) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pituitary gland tissue with recombinant ACTH antibody (clone rCLIP/3814) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Further Information

Application Details :
ELISA (For coating order BSA-free format),Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant ACTH antibody should be determined by the researcher.
Description:
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant protein fragment from the N-terminal region of human ACTH was used as the immunogen for the recombinant ACTH antibody.
Limitation:
This recombinant ACTH antibody is available for research use only.
Localization:
Cytoplasm, Secreted
Purity:
Protein A/G affinity
Species Reactivity :
Human, Mouse and Rat (broad species reactivity predicted)
Uniprot #:
P01189