Ribonucleotide Reductase M1 Antibody / RRM1

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NSJ Bioreagents
Product Code: NSJ-V8809
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8809-20UG20ug£264.00
Quantity:
NSJ-V8809-100UG100ug£534.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: RRM1/4372R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the RRM1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RRM1 antibody (clone RRM1/4372R). These results demonstrate the foremost specificity of the RRM1/4372R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
IHC staining of FFPE human small intestine tissue with RRM1 (clone RRM1/4372R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Negative control: IHC staining of FFPE human brain tissue using RRM1 antibody (clone RRM1/4372R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free RRM1 antibody (RRM1/4372R) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RRM1 antibody (clone RRM1/4372R). These results demonstrate the foremost specificity of the RRM1/4372R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human small intestine tissue with RRM1 (clone RRM1/4372R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using RRM1 antibody (clone RRM1/4372R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free RRM1 antibody (RRM1/4372R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the RRM1 antibody should be determined by the researcher.
Description:
Ribonucleotide reductase M1 polypeptide (RRM1) is one of two non-identical subunits for ribonucleoside-diphosphate reductase, an enzyme which catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. RRM1 is present throughout the cell division cycle but downregulated in quiescent cells. RRM1 is involved in carcinogenesis, tumor progression, and the response of non-small-cell lung cancer (NSCLC) to chemotherapy.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 700-800 of human RRM1 was used as the immunogen for the RRM1 antibody.
Limitation:
This RRM1 antibody is available for research use only.
Localization:
Cytoplasm
Microarray Validated?:
Yes
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
IDP23921