Actin Antibody / Muscle Specific

NSJ Bioreagents
Product Code: NSJ-V8843
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8843-20UG20ug£264.00
Quantity:
NSJ-V8843-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rMSA/953
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 2
Western blot testing of human skeletal muscle tissue lysate using Muscle Actin antibody (clone rMSA/953). Predicted molecular weight ~45 kDa.~
2 / 2
SDS-PAGE analysis of purified, BSA-free Muscle Actin antibody (clone rMSA/953) as confirmation

Western blot testing of human skeletal muscle tissue lysate using Muscle Actin antibody (clone rMSA/953). Predicted molecular weight ~45 kDa.~
SDS-PAGE analysis of purified, BSA-free Muscle Actin antibody (clone rMSA/953) as confirmation

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-2ug/ml
Application Note:
Optimal dilution of the Muscle Actin antibody should be determined by the researcher.
Description:
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant protein was used as the immunogen for the Muscle Actin antibody.
Limitation:
This Muscle Actin antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P62736, P68032 & P68133