EpCAM Antibody / Cytoplasmic domain

NSJ Bioreagents
Product Code: NSJ-V8850
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8850-20UG20ug£264.00
Quantity:
NSJ-V8850-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: EGP40/7035R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human brain tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (EGP40/7035R) as confirmation of integrity and purity.

IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (EGP40/7035R) as confirmation of integrity and purity.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
Description:
Epithelial cellular adhesion molecule (Ep-CAM), also called epithelial specific antigen (ESA), is a 40-43kDa transmembrane epithelial glycoprotein. It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 289-314 of human EpCAM was used as the immunogen for the recombinant EpCAM antibody.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity
Species Reactivity :
Human
Uniprot #:
P16422