Lambda Light Chain Antibody

NSJ Bioreagents
Product Code: NSJ-V8873
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8873-20UG20ug£264.00
Quantity:
NSJ-V8873-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: LLC/3774R
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant Lambda Light Chain antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain antibody (clone LLC/3774R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain (clone LLC/3774R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Lambda Light Chain antibody (clone LLC/3774R) as confirmation of integrity and purity.

IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain antibody (clone LLC/3774R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with recombinant Lambda Light Chain (clone LLC/3774R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Lambda Light Chain antibody (clone LLC/3774R) as confirmation of integrity and purity.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Lambda Light Chain antibody should be determined by the researcher.
Description:
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human IGL protein was used as the immunogen for the recombinant Lambda Light Chain antibody.
Limitation:
This recombinant Lambda Light Chain antibody is available for research use only.
Localization:
Cell Surface, cytoplasmic and secreted
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P01701 & P01842