Topoisomerase II alpha Antibody / TOP2A

NSJ Bioreagents
Product Code: NSJ-V8971
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V8971-20UG20ug£264.00
Quantity:
NSJ-V8971-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rTOP2A/6629
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC staining of FFPE human lymph node with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629) as confirmation of integrity and purity.

IHC staining of FFPE human lymph node with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Topoisomerase II alpha antibody (clone rTOP2A/6629) as confirmation of integrity and purity.

Further Information

Application Details :
Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the recombinant Topoisomerase II alpha antibody should be determined by the researcher.
Description:
Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and re-ligating the broken strand. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 1352-1493 was used as the immunogen for the recombinant Topoisomerase II alpha antibody.
Limitation:
This recombinant Topoisomerase II alpha antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P11388