p27 Antibody / p27Kip1

Contact us
NSJ Bioreagents
Product Code: NSJ-V9161
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9161-20UG20ug£264.00
Quantity:
NSJ-V9161-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rKIP1/1356
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 2
IHC staining of FFPE human breast carcinoma tissue with recombinant p27Kip1 antibody (clone rKIP1/1356). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
❮❯

IHC staining of FFPE human breast carcinoma tissue with recombinant p27Kip1 antibody (clone rKIP1/1356). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~

Documents

Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant p27Kip1 antibody should be determined by the researcher.
Description:
p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-beta-induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human CDKN1B/p27Kip1 protein was used as the immunogen for the recombinant p27Kip1 antibody.
Limitation:
This recombinant p27Kip1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P46527