Ki-67 Antibody

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NSJ Bioreagents
Product Code: NSJ-V9265
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9265-20UG20ug£264.00
Quantity:
NSJ-V9265-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: MKI67/4945R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant Ki-67 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC staining of FFPE human tonsil tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human lymph node tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human Raji and Ramos cell lysates using recombinant Ki-67 antibody (clone MKI67/4945R).
5 / 5
SDS-PAGE analysis of purified, BSA-free recombinant Ki-67 antibody (clone MKI67/4945R) as confirmation of integrity and purity.
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IHC staining of FFPE human tonsil tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant Ki-67 antibody (clone MKI67/4945R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji and Ramos cell lysates using recombinant Ki-67 antibody (clone MKI67/4945R).
SDS-PAGE analysis of purified, BSA-free recombinant Ki-67 antibody (clone MKI67/4945R) as confirmation of integrity and purity.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Ki-67 antibody should be determined by the researcher.
Description:
Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 2293-2478 from the human protein was used as the immunogen for the recombinant Ki-67 antibody.
Limitation:
This recombinant Ki-67 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P46013