MUC1 Antibody / Mucin-1

NSJ Bioreagents
Product Code: NSJ-V9266
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9266-20UG20ug£264.00
Quantity:
NSJ-V9266-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: MUC1/4416R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant Mucin-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human MCF-7 cell lysate using recombinant Mucin-1 antibody (clone MUC1/4416R). This glycoprotein is commonly visualized between 120~500 kDa.
2 / 4
IHC staining of FFPE human breast carcinoma tissue with recombinant Mucin-1 antibody (clone MUC1/4416R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human breast carcinoma tissue with recombinant Mucin-1 antibody (clone MUC1/4416R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant Mucin-1 antibody (clone MUC1/4416R) as confirmation of integrity and purity.

Western blot testing of human MCF-7 cell lysate using recombinant Mucin-1 antibody (clone MUC1/4416R). This glycoprotein is commonly visualized between 120~500 kDa.
IHC staining of FFPE human breast carcinoma tissue with recombinant Mucin-1 antibody (clone MUC1/4416R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with recombinant Mucin-1 antibody (clone MUC1/4416R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Mucin-1 antibody (clone MUC1/4416R) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Mucin-1 antibody should be determined by the researcher.
Description:
Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-Mucin-1 antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently Mucin-1 negative, so keratin positivity with negative Mucin-1 favors one of these tumors. Mucin-1 is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to Mucin-1 is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human MUC1 protein was used as the immunogen for the recombinant Mucin-1 antibody.
Limitation:
This recombinant Mucin-1 antibody is available for research use only.
Localization:
Cytoplasmic, Cell Surface
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P15941