HSP60 Antibody

NSJ Bioreagents
Product Code: NSJ-V9271
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9271-20UG20ug£264.00
Quantity:
NSJ-V9271-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rHSPD1/6495
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant HSP60 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6495). Predicted molecular weight: ~60 kDa.
2 / 3
IHC staining of FFPE human thyroid tissue with recombinant HSP60 antibody (clone rHSPD1/6495). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone rHSPD1/6495) as confirmation of integrity and purity.

Western blot testing of human Hep2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6495). Predicted molecular weight: ~60 kDa.
IHC staining of FFPE human thyroid tissue with recombinant HSP60 antibody (clone rHSPD1/6495). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone rHSPD1/6495) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.
Description:
Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human HSPD1 protein was used as the immunogen for the recombinant HSP60 antibody.
Limitation:
This recombinant HSP60 antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P10809