HSP27 Antibody / HSPB1

NSJ Bioreagents
Product Code: NSJ-V9277
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9277-20UG20ug£264.00
Quantity:
NSJ-V9277-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Recombinant Antibody
Antibody Clone: HSPB1/6490R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the recombinant HSP27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of human liver tissue lysate using recombinant HSP27 antibody (clone HSPB1/6490R). Predicted molecular weight: 23-27 kDa.
2 / 3
IHC staining of FFPE human thyroid with recombinant HSP27 antibody (clone HSPB1/6490R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/6490R) as confirmation of integrity and purity.

Western blot testing of human liver tissue lysate using recombinant HSP27 antibody (clone HSPB1/6490R). Predicted molecular weight: 23-27 kDa.
IHC staining of FFPE human thyroid with recombinant HSP27 antibody (clone HSPB1/6490R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/6490R) as confirmation of integrity and purity.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant HSP27 antibody should be determined by the researcher.
Description:
HSP27, also referred to as Heat shock protein beta-1 (HSPB1), Estrogen-Regulated 24K protein, and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length HSP27 protein was used as the immunogen for the recombinant HSP27 antibody.
Limitation:
This recombinant HSP27 antibody is available for research use only.
Localization:
Predominantly cytoplasmic with some nuclear
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P04792