CD38 Antibody

NSJ Bioreagents
Product Code: NSJ-V9296
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9296-20UG20ug£264.00
Quantity:
NSJ-V9296-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG2a, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rCD38/6447
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CD38 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human Raji cell lysate using CD38 antibody (clone rCD38/6447).
2 / 5
IHC staining of FFPE human tonsil tissue with CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human prostate adenocarcinoma tissue with CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
SDS-PAGE analysis of purified, BSA-free CD38 antibody (rCD38/6447) as confirmation of integrity and purity.
5 / 5
Negative control: IHC staining of FFPE human brain tissue using CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Raji cell lysate using CD38 antibody (clone rCD38/6447).
IHC staining of FFPE human tonsil tissue with CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate adenocarcinoma tissue with CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free CD38 antibody (rCD38/6447) as confirmation of integrity and purity.
Negative control: IHC staining of FFPE human brain tissue using CD38 antibody (clone rCD38/6447) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the CD38 antibody should be determined by the researcher.
Description:
CD38 is a type II transmembrane glycoprotein that is present on early B- and T-cell lineages and activated B- and T-cells but is absent from most mature resting peripheral lymphocytes. CD38 is found on thymocytes, pre-B cells, germinal center B-cells, mitogen-activated T-cells, monocytes and Ig-secreting plasma cells. CD38 is expressed on CD34+ cells. The CD34+CD38- population of hematopoietic stems cells define the most pluripotent cells (e.g.blast colony forming cells).
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 200-300 of human CD38 protein was used as the immunogen for the CD38 antibody.
Limitation:
This CD38 antibody is available for research use only.
Localization:
Cell Surface, Cytoplasm
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
P28907