Acidic Cytokeratin Antibody (LMW / Type I)

NSJ Bioreagents
Product Code: NSJ-V9300
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-V9300-20UG20ug£264.00
Quantity:
NSJ-V9300-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: rKRTL/6616
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Aliquot the recombinant Acidic Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human skin tissue with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Acidic Cytokeratin antibody (clone rKRTL/6616) as confirmation of integrity and purity.

IHC staining of FFPE human sebaceous gland with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin tissue with recombinant Acidic Cytokeratin antibody (clone rKRTL/6616). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Acidic Cytokeratin antibody (clone rKRTL/6616) as confirmation of integrity and purity.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant Acidic Cytokeratin antibody should be determined by the researcher.
Description:
The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK10, CK14, CK15 and CK16 and low molecular weight CK19. Its usually used in a cytokeratin cocktail with type II cytokeratin antibody.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A synthetic peptide sequence common to Type I Cytokeratins was used as the immunogen for the recombinant Acidic Cytokeratin antibody.
Limitation:
This recombinant Acidic Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Species Reactivity :
Human
Uniprot #:
Q7Z794